RNA
Part:BBa_K3001013:Design
Designed by: Sydnee Calhoun Group: iGEM19_Lethbridge_HS (2019-10-12)
Lwa crRNA targeting GFP with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A T7 promoter was added to allow overexpression in E. coli BL21(DE3) or Rosetta (DE3) cells or for in vitro transcription. A double terminator was also added to signal the end of transcription.
Source
Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.